Hair Tissuelyser
Frozen Tissue Homogenization DHFSTPRP-CL24 hair homogenization a special vertical up-down integrated vibration mode is adopted, through the high-frequency reciprocating vibration, impact and shearing of grinding beads (zirconia, steel balls, glass beads, ceramic beads). The ideal grinding effect can be achieved quickly, so that the ground samples have more sufficient, more uniform, better sample repeatability, and no cross-contamination between samples. After grinding, it cooperates with the technical specification for forensic identification SF/Z JD0107025--2018 "Liquid chromatography-tandem mass spectrometry test method for 15 drugs and metabolites in hair", which can detect common drugs in hair (morphine, methamphetamine, ketamine, ecstasy, marijuana) etc.) for on-site rapid testing.
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Frozen Tissue Homogenization DHFSTPRP-CL24 hair homogenization a special vertical up-down integrated vibration mode is adopted, through the high-frequency reciprocating vibration, impact and shearing of grinding beads (zirconia, steel balls, glass beads, ceramic beads). The ideal grinding effect can be achieved quickly, so that the ground samples have more sufficient, more uniform, better sample repeatability, and no cross-contamination between samples. After grinding, it cooperates with the technical specification for forensic identification SF/Z JD0107025--2018 "Liquid chromatography-tandem mass spectrometry test method for 15 drugs and metabolites in hair, which can detect common drugs in hair (morphine, methamphetamine, ketamine, ecstasy, marijuana) etc.) for on-site rapid testing. The detection limits of various drugs have reached the requirements of the "Specifications for the Detection of Hair Samples of Drug-Involved Persons" issued by the Ministry of Public Security. The product has been widely recognized in the industry since its inception, and has obtained a number of innovations, and has obtained the acceptance report of the key laboratory of the Beijing Municipal Public Security Bureau for court toxic analysis and the Ministry of Public Security.
This instrument is a recommended product of the Forensic Identification Technical Specification SF/Z JD0107025--2018 "Liquid chromatography-tandem mass spectrometry detection method of 15 drugs and metabolites in hair
Rationale:
Hair samples were washed, frozen and ground, extracted with methanol by ultrasonic method, and detected by liquid chromatography-tandem mass spectrometry in multiple reaction monitoring (MRM) mode. Qualitative analysis was carried out in terms of retention time, mass spectral characteristic fragment ion peaks and relative abundance ratios of ion pairs by comparing with blank samples and spiked samples operated in parallel.
DHFSTPRP Series--Frozen Hair Grinder Features:
○ Efficient Grinding—
Realize the rapid on-site grinding of 24 sample hairs at the same time. The grinding equipment has a built-in program controller, which is easy to operate. At the same time, it provides feasible solutions for community drug rehabilitation and special personnel monitoring.
○ Cryogenic Grinding -
The freezing grinding temperature can be adjusted according to your needs, and the sample components are protected by low temperature, and the sample components will not be destroyed.
○ Professional Grinding—
The instrument has completed the detection of nearly 1,000 real samples in judicial centers and anti-drug teams in Beijing, Ningbo, Shanghai and other places. The positive detection rate is as high as 99%, effectively avoiding false positive interference caused by taking drugs.
Grindable samples:
| 1. Plant tissue: | roots, stems, leaves, flowers, fruits, seeds, etc. |
| 2. Animal tissue: | Hair, Brain, Heart, Hair, Lungs, Stomach, Liver, Thymus, Kidneys, Intestines, Lymph Nodes, Muscles, Bones, etc. |
| 3. Fungal bacteria: | Yeast, Escherichia coli, etc. |
| 4. Food and Drugs: | All kinds of food, pills, etc. |
| 5. Volatile samples: | Coal, oil shale, wax products, etc. |
| 6. Plastics, polymers: | PE, PS, textile, resin, etc. |
·Extract nucleic acid/protein
Cryogenic grinding of samples can effectively inhibit nucleic acid degradation and retain protein activity.
·Analysis of active ingredients
Significant differences often exist between isomers of active pharmaceutical ingredients. Cryogenic grinding prevents molecular degradation due to pressure and heat.
Reduce sample volatilization
Cryogenic grinding can significantly reduce the volatilization of the sample and preserve the content of the sample components more completely
Grinding tough/hard samples
For difficult-to-grind samples such as high-toughness plastics, high-strength plastics, and resins, cryogenic grinding can greatly improve the grinding effect and efficiency.
Frozen Tissue Homogenization DHFSTPRP-CL24 hair homogenization a special vertical up-down integrated vibration mode is adopted, through the high-frequency reciprocating vibration, impact and shearing of grinding beads (zirconia, steel balls, glass beads, ceramic beads). Quickly realize the grinding effect, so that the ground samples have more sufficient, more uniform, better sample repeatability, and no cross-contamination between samples.
Technical Specification for Forensic Identification SF/Z JD0107025–2018 Liquid chromatography-tandem mass spectrometry detection method of 15 drugs and metabolites in hair
6 how-tos
6.1 Sample Preparation
6.1.1 Case Sample
The hair samples were washed twice with appropriate amount of water and acetone in sequence, dried and cut into pieces of about 1mm, which were pulverized in a refrigerated grinder to form a powder.
Weigh 20 mg of hair powder, add 1.0 mL of internal standard methoxyphenamine standard working solution (1 ng/mL methoxyphenamine), ultrasonicate in ice bath for 30 min, centrifuge, pipette The supernatant was dried under air flow (or dry nitrogen blower) in a water bath at 60°C. The residue was reconstituted with 100 μL of methanol for instrumental analysis.
6.1.2 Blank sample
Take 20mg of blank hair sample, add 1.0mL internal standard methoxyphenamine standard working solution (1ng/mL methoxyphenamine), the rest is the same as 6.1.1, and the case sample Parallel extraction operations.
6.1.3 Add samples
Take 20mg of blank hair sample, add the suspicious target reference substance in the case sample, add 1.0mL internal standard methoxyphenamine standard working solution (methoxyphenamine 1ng/ mL), the rest is the same as 6.1.1, and the extraction operation is performed in parallel with the case sample.
| The number of operations is large, the effect is good | Efficient and fast work can complete the grinding of 2×24, 2×48, 4×96 samples in 1 minute. Save time and effort, and the difference between batches and batches is small. The extracted protein has higher specific activity and longer nucleic acid fragments. |
| No cross contamination | The sample tube is completely closed during the crushing process, and disposable centrifuge tubes and beads can be used. The sample remains intact in the tube to avoid cross-contamination between samples and external contamination. |
| Easy to operate | 1. Built-in program controller, which can set parameters such as grinding time and rotor vibration frequency; 2. Humanized operation interface. |
| good stability | 1. The vertical oscillation method is adopted, and the grinding is more sufficient and the stability is better; 2. During the operation of the instrument, the noise is less than 55dB, which will not interfere with other experiments or instruments. |
| Convenient low temperature operation | When an ultra-low temperature grinding environment is required, the adapter with the sample can be immersed in liquid nitrogen to cool for 1-2 minutes. After taking it out, it can be moved to the host for quick fixation, and grinding can be started without re-freezing, which is convenient and quick. |
| good repeatability | The same tissue sample was set to the same program to obtain the same grinding effect. The working time is short and the sample temperature does not rise. |
| Applications | Hair grinding, tissue grinding, homogenization, cell disruption, homogenization, material dispersion, preparation, sample mixing, shaking, etc. |
| Throughput | The maximum processing capacity within 15 seconds can process 24 samples at the same time, integrated compressor refrigeration |
| Compatible sample books | 24*(0.2-0.5ML) /24*2ML /12*(5-15)ML /4*25ML /2*50ML, various specifications of grinding tubes can be customized |
| Display method | Touch screen display for convenient and intuitive operation |
| Data Storage | Ten groups of experimental data can be stored. According to different experimental samples, animal heart, spleen, lung, kidney, bone, skin, and hair patterns are set. |
| Pattern loop | According to the set experimental parameters, it can continuously cycle between several set parameters to further reduce the interference of human factors |
| Maximum feed size | No requirement, adjust according to adapter |
| Cooling function | Yes, adjustable from -30 degrees to 37 degrees |
| Temperature control accuracy | + 0.5℃ |
| Open cover operation protection | Electromagnetic lock |
| Final discharge particle size | ~5μm |
| Number of grinding platforms | (Number of acceptable grinding jars)>2 |
| Fastening device | With automatic centering |
| Homogenization speed | 0～70 Hz/sec, working time: 0sec～99min, user can set by himself |
| Grinding Ball Diameter | 0.1～30mm |
| Grinding Ball Material | Alloy steel, chrome steel, zirconia, tungsten carbide, quartz sand |
| Accelerate | Reach max speed in 2 seconds |
| slow down | Reach minimum speed in 2 seconds |
| Noise level | <55db |
| Grinding method | Wet grinding, dry grinding, cryogenic grinding are available |
| Thermostat can be reconfigured | With the ability to upgrade to ultra-low temperature liquid nitrogen refrigeration or air refrigerator refrigeration |
| Adapter Material | PTFE, alloy steel |
| Safety Instructions | Fastening device with automatic centering, safety lock when working, full protection |
| Grinding Kit Materials | Rigid steel, , PTFE (Teflon), zirconia |
| Dimensions | 630*300*480mm |
| Weight | 58 Kg |
| host | 1 set |
| adapter | ?2ml 1set |
| Refrigeration adapter | ?2ml 1set or ?1.5ml 1set（2 out of 1） |
| grinding beads | 1. 1000 ?5 imported stainless steel grinding beads or 1000 ?5 imported ceramic grinding beads (choose 1 from 2); 2. 1000 ?3 imported stainless steel grinding beads. |
| Special Grinding Tube | 200 piece |
| Special bead gun | 1 set（Optional） |
1. The matching LW kit can be used to quickly, efficiently, stably, and batch extract DNA/RNA proteins from samples.
2. 1Hz=30 revolutions/second / 1Hz=100RPM
3. Conventional adapters can be refrigerated to give samples a cool environment while working. It can be equipped with an adapter with refrigeration, which can be soaked in liquid nitrogen for more than 30 minutes.
4. The noise emission value for the working environment depends on the type of sample and the settings of the grinder. The parameters in the table are in the no-load state.
5. Small samples typically used for DNA and RNA isolation can be prepared in disposable centrifuge tubes. Adapters made of Teflon material can be used to accommodate 5 or 10 disposable sample tubes for cell disruption. Cells and tissues are rapidly and efficiently disrupted in a very short time in the Multi-Sample Tissue Grinder, thus eliminating the need for additional cryogenic refrigeration.
